xdna files from Serial Cloner, which is an extended version of the old DNAStrider format.Įnhancements to Outlining Shared Domains in Aligned Sequencesįirst added in MacVector 17.5, this functionality has been significantly enhanced to expose additional Feature-related functionality in Multiple Sequence Alignment documents. spf Assembly Project files from Sequencher dna files, complete with feature colors, like this plasmid downloaded from the AddGene website The results are shown in the Map tab, and you can zoom in to see the actual sequence of the hits with the guide sequence shown in lower case and the PAM sequence in upper case You can also have MacVector automatically scan for CRISPR PAM sites whenever you open a DNA sequence file - control this using the MacVector | Preferences | Scan DNA pane. The output is a mix between the current Restriction Enzyme and Nucleic Acid Subsequence searches, so you can easily see and copy the guide sequences. While MacVector does not currently search genomes for potential off-target sites, this is still a very useful function to alert you to potential CRISPR target sites. It contains most of the current Dec 2020) characterized Cas9-like enzymes, but you can always open the file and add your own. By default, MacVector uses a file called Protospacer Adjacent Motifs.pam that is located in the / Applications/MacVector/Subsequences/ folder. menu item that will scan Nucleic Acid sequences for Protospacer Adjacent Motifs associated with the CRISPR Cas9 and related enzymes cleavage and modification functions. There is a new feature accessed by the Analyze | CRISPR PAM sites. Otherwise, MacVector 18 has the identical functionality to MacVector 18.1 but with a slightly smaller disk footprint. We recommend this for all users of Intel-base Macintosh computers and for users still unning OS X 10.11 (El Capitan). It requires a minimum of macOS 10.12 (macOS Sierra). MacVector 18.1 is a Universal Binary, meaning that it runs natively on both Intal and Apple Silicon ("M1") Macintosh computers. Finally, there are major enhancements to the protein multiple sequence alignment visualization of domains and the usual macOS compatibility enhancements. There is a new CRISPR PAM sequence searching function and a Trim by Quality option for Sanger sequence files. MacVector 18 adds a number of new functions, including the ability to read a several requested file formats such as Sequencher assembly project files along with Serial Cloner and SnapGene sequence files. ![]() Sequence Analysis Tools for Molecular Biologists
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![]() For the Buttercream Frosting:īutter – it should be softened, not straight from the fridge or melted. Just follow the instructions at the back of the box and you’re all set. Vanilla and Strawberry Cake Mixes – The great thing about these ready-made mixes is that there’s no need to measure ingredients, which means there’s no chance of you messing up the measurements! Here’s what you’ll need to make it: For the Cake: Finally, the cake is coated in crushed freeze-dried strawberries and Nilla wafers. You’ll find vanilla buttercream between the layers, which is also spread on the outside. Strawberry crunch cake is made of two layers of vanilla cake and two layers of strawberry cake. Together, they create the perfect dessert worthy of any special occasion. The crunch from the Nilla wafers also provides a lovely textural contrast to the tender cake. I love how the tartness of the strawberries perfectly balances out the sweetness of the buttercream. Thanks to the strawberries, this cake is refreshing as can be. The recipe uses cake mixes for the base, but don’t worry about it tasting artificial. You could even add a layer of strawberry jam between the cakes if you want a pop of sweetness. While the frosting is still wet, you’ll then add a fruity and crunchy coating which is a blend of crushed freeze-dried strawberries and Nilla wafers. That same frosting is then used to cover the whole cake. They’re then sandwiched together with American buttercream frosting. Strawberry crunch cake is a wonderful layer cake made with layers of strawberry and vanilla sponge cakes. The texture on the outside makes a nice contrast to the soft cake and creamy frosting. That could be nuts, coconut, crushed cookies, or freeze-dried fruits. What Is A Crunch Cake?Ī crunch cake is a cake that’s frosted or glazed and then coated with a layer of crunchy ingredients. What’s not to love?Īnd when it looks this good, nobody will bat an eye at how you did it. With four cake layers filled with buttercream frosting and covered in a scrumptious strawberry crunch, this cake is what strawberry dreams are made of.Īs a baker, I can honestly say that I often turn to boxed cake mixes quite often.Īfter all, they come out perfect every single time, and they take little to no effort. Phenomenal is not enough to describe the strawberry crunch cake. So nobody has to know you took a shortcut! Strawberry Crunch Cake This strawberry crunch cake still tastes fantastic. Why? Because you won’t be baking from scratch.īut don’t worry. While frosting a cake can be quite a challenge, the baking part is still relatively easy. ![]() ![]() If it’s your first time baking a layer cake recipe, this is a good place to start. The main purpose of this program is to keep you away from junk and Trojans file. If you want to keep safe from the effect of danger system you need a PC Cleaner Pro 2018 Crack for your protection. 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PC Cleaner Pro v14.0 License Key (Mac + Windows) Free PC Cleaner Pro License Key makes your PC faster by boosting startup speed, optimizing registry, as well as removing unwanted applications which make your computer slower. By fixing this missing information in your. Fix Your PC Errors -PC Cleaner Pro will safely scan your Windows registry and finds incorrect or obsolete information. Cc cleaner Professional v FINAL + Serials.exe 4.11 MB. ![]() Claves_De_Licencia_Onesafe_Pc_Cleaner_5JJ07L.exe 1.24 MB. In order activate OneSafe PC Cleaner please enter the licence key you've bought, after pressing on the cog icon, then press on Register. ![]() The salon regulars are fighting over a card game in which they’ve been gambling for pennies, and an accusation of cheating has them at each other’s throats. ![]() He works as an assistant investigator, and today he and his partner JI-HOON ( Nam Yoon-soo) are responding to a disturbance at a local hair salon. LEE DONG-SHIK ( Shin Ha-kyun) is all grown up now, and he appears to be living in the old remains of that cafe, which is now wallpapered with articles about Joo-seon’s murder and his missing sister. When Dong-shik and Yoo-yeon’s mother steps outside to get the paper, she’s horrified to see Yoo-yeon’s fingertips neatly lined up on the doorstep, but Yoo-yeon is never seen again. In the morning, Joo-seon’s body lies in the reed field, her ankles and wrists tied together and her fingertips cut off. Suddenly she hears a woman screaming, and the streetlights go out. She heads out, ending up on a long road near a reed field. Later that night, Dong-shik’s sister Yoo-yeon gets a text from Dong-shik saying that he doesn’t want to go home, and asking her to meet him elsewhere. Dong-shik goes, but before he does, he tells her, “Bang Joo-seon, see you later…” with an unsettling smile that doesn’t quite reach his eyes. Eventually the owner’s daughter gets fed up with the noise and orders Dong-shik to leave, grumbling that he’s nothing compared to his sister. Mom tries to call him, but LEE DONG-SHIK (cameo by Lee Do-hyun) is too busy loudly and badly playing guitar and singing along with the radio at a nearby café. Unfortunately, Yoo-yeon’s older twin brother seems to be the complete opposite, and didn’t even show up for today’s mass for his own grandmother, though his mother faithfully defends him. In the same town, Munju City, a young woman named LEE YOO-YEON ( Moon Joo-yeon) plays the organ at church while her mother humbly accepts compliments from a fellow church member about what a sweet, thoughtful, and smart daughter she is. The first man finds him by his flashlight, and gapes at something on the ground - a pair of mummified hands with the fingertips cut off. A younger man calls back that he fell but he’s okay, but then his flashlight falls on something that makes him start screaming. NOTE: This is only a first episode recap.Ī man wanders through a reed field at night, calling for someone. They’re forced to work together against their wishes - will their suspicions and hangups get in the way of solving a murder spree that’s lasted for over two decades? ![]() A small-town murder hits close to home for one of them, while the other seems determined to solve the case even if it puts him in harm’s way. Yeo Jin-gu and Shin Ha-kyun are teaming up for Beyond Evil, a murder mystery that seems poised to be a fantastically shivery thrill ride. 72 FebruFebruBeyond Evil: Episode 1 by LollyPip ![]() Services will be offered at more than 4,700 Walmart stores with a rollout planned to begin in the spring of 2021. The services will include domestic and international money transfers, bill payments and money orders. bringing greater choice and value to millions of customers. Walmart, the world’s largest retailer, and Western Union, a global leader in cross-border, cross-currency money movement and payments, today announced a new agreement that will, for the first time, enable Western Union money transfer services at Walmart locations across the U.S. Walmart and Western Union Enter Agreement to Offer Western Union Money Transfers at Walmart. Through this partnership, Nationwide pet insurance members will have access to Walmart’s most commonly prescribed pet-specific medications for chronic disease and flea, tick and heartworm prevention. ![]() Preferred pricing and the opportunity to have claims for covered drugs processed at the counter of a major retail pharmacy is a first for the pet health insurance industry. Members can log into my. to sign up for the program and access their digital ID card to present at the Walmart pharmacy. Nationwide Pet Rx ExpressSM gives Nationwide’s pet insurance members access to affordable pet prescription medicines and introduces a new, expedited, in-store claims experience. Nationwide and Walmart, the nation’s largest pet insurer and the nation’s largest retailer, are joining forces to help families with pets save time and money when filling their pet prescription medications at any of Walmart’s 4,700 pharmacies across the country. Nationwide Pet Rx Express offers pet insurance members savings, added convenience with streamlined claims process at Walmart pharmacies Nationwide® partners with Walmart on first-of-its kind pet prescription program Love Better" - Your local Super Walmart location We're a Super Walmart located at 18185 Zane St Nw in Elk River, MN. ![]() In some cases, a decrease in academic performance was found. ![]() While some studies found a significant increase in student grades after introducing lecture capture, others reported no or only marginal effects. Whether lecture capture actually improves student learning and attainment is, however, still a matter of debate. Many students perceive lecture capture as important to their studies and think that it helps them to achieve better grades. They also report that lecture capture makes learning easier, for example by facilitating note taking and helping with revision. Students like the flexibility and convenience associated with the availability of lecture recordings. Undoubtedly, lecture capture is popular with students. In addition, the technology plays a role in creating inclusive curricula, for example by helping to overcome language barriers and by supporting students with dyslexia and other specific learning disabilities. found that lecture capture reduces student anxiety by providing a supportive environment to some students. Many studies have investigated the benefits and drawbacks of lecture capture. In addition, factors such as high student numbers, inadequate funding and the increased need for students to work while studying may also play a role in an institution's decision to introduce systematic recordings of lectures. universities are under pressure from students to adopt web‐based lecture technologies such as lecture capture. Lecture capture is becoming increasingly commonplace in Higher Education settings in the UK and worldwide. The term ‘lecture capture’ refers to the real‐time recording of live (face‐to‐face) lectures, simultaneously recording audio signals such as the instructor's voice as well as images (such as PowerPoint slides) and sound on the computer (here, the term ‘lecture capture’ does not include pre‐recorded lectures used for blended/online and/or flipped learning). The outcomes of our study suggest that lecture capture provision may negatively affect some students' attendance and study behaviour, and thus, we suggest more needs to be done to mitigate against this. ![]() However, about one‐third of the participants reported skipping more classes, and the same participants were more likely to postpone catching up on missed lectures. For most students, lecture capture did not affect self‐reported live lecture attendance patterns. For many, lecture capture seems to reinforce the view that memorisation equals learning, a view that may be reinforced by knowledge‐focussed assessment formats. Students reported that they spend a considerable amount of time watching recordings and making verbatim notes, leaving them little time for independent study. The participants in our study were convinced that lecture capture improved their learning, and many students noted that they were dependent on the recordings, thinking that without them, they would not be able to achieve good grades. Here, we performed a survey ( n = 694, 53% of the cohort) and set up focus groups (2 focus groups, 15 participants) to explore biological sciences students' perceptions of how lecture capture impacts their study behaviour when recordings are provided for every lecture and are made available to students without restriction. It is popular with students who like the associated flexibility and believe that lecture recordings improve their grades. Lecture capture (the real‐time recording of live lectures) has become commonplace in higher education. It is the one I'd prefer between the two choices here, simply because of the Invest in Stock ability. The Investor Branch has an Ideal Mood of Focused. Investor Career Sims 4's Investor Branch offers Focused Items Make Business Calls, Reach Level 10 Charisma, Level 8 Logic $1074, Executron Bookshelf, New CAS Parts Make Business Calls, Reach Level 8 Charisma, Level 6 Logic ![]() Make Business Calls, Reach Level 6 Charisma, Level 5 Logic In order to get Confident before Work, you can simply Psych Self Up using a mirror or make your Sim brush their teeth. I'd do them after work so that there is no chance of going into work totally embarrassed and risking some kind of freak accident emotional death. It's more important you complete them than if you bother to make them. Sims who make successful calls may get a Confident or +2 Embarrassed Moodlet depending whether they succeed or fail at the deal. Making these calls can help you get promoted faster so long as you have the Skill requirements met. They may, however, help with the success rate of Make Business Calls (found on the Phone under Household), which may only be done from 8PM-8AM on Weekdays - meaning your Sim needs to do them after work hours. The Management Career Branch features Confident Aura items, which are appropriate for the Career however not very useful for gaining progress in it because they don't work when you're out of the room. Check out my many mods here! Management Branch Rewards offer Confident Auras $281, Pie Chart Presentation, Offer Career Advice Interaction, New CAS PartsĮxplore My Mods! Color Picker for Builders, Seasons Tuner, Dine Out Reloaded and a Gameplay Overhaul! Like other modders, I've been working to make The Sims 4 a better game. $130, Desk Nameplate (Stolen), Make Fun of Corporate Goons Interaction, New CAS Parts ![]() Gossip about Office Romances Interactions Just accept that your Sim has less time in the early levels and will eventually get a better job without the boring paperwork. This is tough, as it takes a few hours to do and you're already gone for the 9-5. Filling out Reports may sometimes give your Sim a Bored Moodlet, "Paperwork Drudgery". The starting Daily Task, Fill out Reports, can be found on the Computer under the Web menu. You can now get promotions with careers.promote business - you can also see all Career Cheats or visit a full list of Cheats in The Sims 4. Press Control + Shift + C then type testingcheats on and press enter. ![]() Green food dye: You can leave the food dye out of the recipe if you like, but I love to add it so everyone can easily tell they’re key lime cheesecakes.Vanilla extract: Use pure vanilla extract for the best flavor.Key lime zest: Make sure to wash your lime before zesting it to remove any dirt or bacteria.Key lime juice: You’re welcome to substitute lime juice for key lime juice, if needed.Sour cream: Breaks up the heaviness of the cream cheese and creates a smoother, creamier filling.Cream cheese: It’s b est to use bricks of cream cheese and avoid cream cheese spread, which has ingredients added to it to thin it out.Granulated sugar: Used to sweeten both the crust and the cheesecake filling.Butter: I recommend using unsalted butter since the amount of salt in salted butters can vary between different brands.Graham crackers: You can pulse full sheets of graham crackers in a food processor to make your own graham cracker crumbs, or you can purchase pre-made graham cracker crumbs.Here’s what you’ll need to make these mini cheesecake bites: These mini key lime pie cheesecakes have three components: the graham cracker crust, the key lime cheesecake filling, and the optional whipped cream on top. Between the buttery graham cracker crust, creamy key lime cheesecake filling, and the homemade whipped cream on top it was almost impossible not to eat the whole batch. Since March is finally here and I have all things fruit on the brain, I decided to create a mini key lime cheesecake version. I mean all desserts are better in mini form, right? I feel like mini cheesecakes are the perfect sized desserts to bring to parties or even just to eat by yourself. So many of you loved these mini Oreo cheesecakes I posted a while back and I have to say, I really loved them too! I’ve been working on a few more variations for mini cheesecakes because they’re so darn cute. The perfect dessert for any time of year! ![]() These Mini Key Lime Cheesecakes feature an easy homemade graham cracker crust topped with a smooth and creamy key lime cheesecake filling. ![]() During those two years, the inseparable pair have shared an incredible bond (and countless dance-offs) for which Li expresses her gratitude. See more of Noble and his family of Angeles-based Terina Li and Kubo, her precious Shiba, are coming up on two years together as pet parent and pup. I’m thankful you were the pup we picked and that you are every bit as weird, loving, and loyal as I am. These past 3 years with you have been one crazy rollercoaster but I wouldn’t have it any other way. You made me stronger, and you made me want to get out and explore. We were so focused on giving you a better life I forgot about my own struggles. Then there you were: 5 weeks old and needing so much love and care. I was scared, homesick and I felt so alone. When you came into my life, I had just moved from middle-of-nowhere Illinois to St Louis. ![]() "Dear Noble: If you only knew just how thankful I am. But Hadley is thankful for all of the adventures the always-loving and loyal Noble has brought her. See more of Opal and her family of the past three years, Shayna Hadley, her daughter, Paisley, and their Dalmatian Noble have been through some pretty big changes together, including a move to Colorado. My heart is bigger because you are a part of it. ![]() Thank you for taking up all the storage space on my phone and for being my icebreaker on dating apps. Who loves unconditionally, uncontrollably, and uncompromisingly. Who has made me an advocate for those without a voice. Who has taught me to bask in the sun, relax in the shade, and roll in the grass. Who is always sad to see me leave and happy when I come home. Who destroys underwear, humps pillows, and chases frogs. You who eats her own poop and is afraid of inanimate objects. Sometimes I wonder if I would have gotten through this past year without you. Four years, three couches, two apartments, one failed engagement-and the only thing I know for certain is you." Four years of waking up to your morning stretches (and sometimes explosive diarrhea). "Dear Opal: It’s been four years since I stumbled across your mismatched ears on Petfinder. Four years after adopting Opal, she is still grateful that their paths crossed on Petfinder. Courtesy of Blanchard lives in West Warwick, Rhode Island, with her rescue dog Opal. ![]() See how clear the picture is! For a webcam, it’s just AMAZING. Logitech Camera Settings (for any Logitech cam, and you can control every detail – lighting, brightening, width etc.).Logitech Capture (with this software you can indeed turn off autofocus, and set your own brightness).The good thing is solve some of the problems downloading the Apps for FREE from Logitech: Warning for MacOS users: if you have an M1 chip, the software is NOT compatible.Many older PCs have just USB2 and USB3 type-A connectors, so you’ll need an adapter if you don’t have any (or many) USB type-C ports. The cable is a bit short and is a USB type-C connector.A wide 78-degree field of view always keeps you up front and center while recording.(Every freckle and wrinkle shows up, even in low light.) Image quality and detail is good enough to be a bit frightening, LOL.You could be in a VERY DIM room, and using the Logitech Capture software, you will look like you have studio lighting! It can make you look like you’re in a brighter room than you actually are.It will focus even very close the cam’s user measured the autofocus minimum working distance at 70 mm.Not exactly in the cheap webcam category (about $150), but this time you definitely get quality for your money. Then my dear hubby bought me this one! Oh God, he is so attentive, well, I like it a lot too. ![]() My old camera used to freeze and blur the images, and I was often nervous and psychotic about it. Since I often participate in conferences, the webcam has become my faithful companion. Logitech StreamCam - for a super clear imageĭon’t think of me as biased, but I just couldn’t help but put this model in this place! I have a very sweet story about it. The options below provide excellent video quality as well as audio quality for your lectures! 1. These tend to be a cheaper option for those on a budget, though they are still certainly an upgrade from the average built-in webcam that comes with your computer. The first category of cameras we have is webcams, which are small external cams that connect to your computer and provide a stream. Teachers and students can agree that a high-quality cam with clear sound quality is essential, so here are our top choices in a wide range of prices! Webcams for Conducting Lessons Online Now that we’ve discussed the benefits of video-based learning and the tops tips for creating engaging videos, let’s get into the cams for recording. Stay tuned, because you don’t want to miss these crucial tips for engaging with your students in video-based learning and choosing the right equipment! 4 Questions for Choosing a Camera for Recording Lectures Question Answerįor pre-recording lectures and making educational videos for your You-Tube channel. Choosing it is a large investment, so it’s not a decision to take lightly. Check out our YouTube channel, get excited!īefore we get into the list of top models, let’s go over some tips for the process of choosing a classroom cam. ![]() More of a fan of watching videos than reading long articles? For you, we’ve made a video about the best cameras for classroom recording. This kindergarten teacher is going viral on TikTok for her videos about online learning ? /B2s9kYepP7 No one can deny the importance of a good camera, even if you’re making some educational TikTok videos!
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